|
KMID : 0545119980080040301
|
|
Journal of Microbiology and Biotechnology
1998 Volume.8 No. 4 p.301 ~ p.304
|
|
|
PHB Accumulation Stimulated by Ammonium Ions in Potassium-limited Cultures of Methylobacterium organophilm
|
|
KIM, SEIN-WON
KIM, PIL/KIM, JUNG HOE
|
|
|
Abstract
|
|
|
|
Methylobacterium organophilum can use nitrogen in the form of ammonium ions ((NH_4)_2SO_4 and NH_4Cl) and from nonammonium sources such as glycine, alanine, peptone, and yeast extract. When potassium was limited, significantly more PHB was produced when the ammonium ion was the nitrogen source rather than a nonammonium form. With ammonium, the amount of PHB produced was 0.50∼0.53g PHB/l or 52.0∼53.2% of the dry cell weight. If nitrogen was from a nonammonium source, the respective values were 0.04∼0.06g PHB/l or 8.1∼11.3% of dry cell weight. When ammonium sulfate was the sole source of nitrogen under potassium-limited conditions, cell growth and PHB accumulation increased as the pH increased from 6.0 to 7.5. Cell growth and PHB amount at pH 7.5 were 2.50g dry cell weight/l and 1.40g PHB/l, respectively.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|